*Please scroll down to the bottom of the page for the final deliverable.
Located in San Diego, BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research.
The objective of this video is to increase sales of the Flex-T product line from the BioLegend website.
Do you need a reagent
that identifies antigen-specific T-cells
and you’re interested in recognizing a specific peptide antigen?
Sounds like an MHC tetramer,
but the conventional method for making tetramers with a specific peptide is a complex and labor-intensive process,
and most commercial tetramers that are preloaded with peptide are expensive
and limited to a small set of predefined antigens.
This is why BioLegend offers the Flex-T technology.
With Flex-T technology, loading your peptide-of-interest onto an MHC monomer is fast and efficient,
and, once tetramerized,
the custom MHC tetramer delivers highly reproducible results.
Loading a peptide is an easy three-step process:
Step 1: Mix your peptide-of-interest with Flex-T monomers.
The Flex-T monomers come pre-loaded with a UV light-labile peptide.
Step 2: Expose your sample to UV light.
The UV light degrades the pre-loaded peptide,
enabling binding of your peptide of interest to the MHC monomer.
Step 3: Mix monomers with fluorophore-streptavidin to create tetramers.
Once formed, the tetramers are ready to use.
Available in a range of MHC alleles,
Flex-T is a powerful, stable, and cost-effective method for studying antigen-specific T-cells for basic research, screening, and more.
Try Flex-T today, order at biolegend.com/order-flex-t.