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Single Domain Antibodies

*Please scroll down to the bottom of the page for the final deliverable.

Abcore is a supplier of antibodies and related products and services to the pharmaceutical reagents market. At the time of this project Abcore’s primary product is VHH single domain antibodies (nanobodies) produced by llama. Biotech Animated was commissioned to create a detailed animation covering the following points:

  • The need in the antibody market nanobodies address.
  • What the features, advantages and benefits of nanobodies are.
  • How they are produced by Abcore (and why llama).
  • What the exit points are for the nanobody production. Abcore specifically

requested that Biotech Animated help easily communicate the hand off points:

  1. Blood serum package (very common for San Diego companies).
  2. PBMC package.
  3. mRNA / cDNA package.
  4. Library package.
  5. Screening package.
  • The call to action is contact Abcore using a webform on the Landing Page.

Additional points about the project are:

  • This Video Presentation was made with the intention of being placed on the Landing Page that Abcore is running Google AdWords to.
  • The goal of the Video Presentation is to increase conversion rate of the PPC advertising campaign and present the product in a more effective and polished way.
  • The target audience is large pharmaceutical companies and antibody companies that want to build out their catalogues.

Storyboard

Abcore Video Storyboard 01

If your scientific research involves the use of antibodies

Abcore Video Storyboard 02

Or if you are a company involved with the antibody reagents market

Abcore Video Storyboard 03

You're probably aware that the field of recombinant antibody technology is rapidly progressing

Abcore Video Storyboard 04

Because of increasing interest in human therapeutic use.

Abcore Video Storyboard 05

Furthermore, an advancing ability to select specific human antibodies

Abcore Video Storyboard 06

And to improve their affinity, stability, and expression level is futher boosting the popularity of this field.

Abcore Video Storyboard 07

But here's the problem with traditional antibodies.

Abcore Video Storyboard 08

They are only stable over a narrow pH and temperature range.

Abcore Video Storyboard 09

And their large size means they are excluded from binding to many active sites on proteins.

Abcore Video Storyboard 10

With this in mind, we at Abcore proudly present to you our flagship product.

Abcore Video Storyboard 11

Single-domain antibodies.

Abcore Video Storyboard 13

Single-domain antibodies are heat stable up to 70 °C.
They are stable over a wide pH range.

Abcore Video Storyboard 14

Compared to traditional antibodies which are approximately 150 kDa in size, they are only 15 kDa.

Abcore Video Storyboard 15

Which means they can recognize targets in active sites that are not accessible to traditional antibodies.

Abcore Video Storyboard 16

Here, you can see the structure of a single-domain antibody compared to a traditional antibody.

Abcore Video Storyboard 17

Notice how the single-domain antibody contains only the antigen-binding variable domain,
also called the VHH domain.

Abcore Video Storyboard 18

Single-domain antibodies also have the following advantages over regular antibodies.

Abcore Video Storyboard 19

They can penetrate into tissue much more rapidly.

Abcore Video Storyboard 20

They are less likely to be immunotoxic.

Abcore Video Storyboard 21

They can easily be converted into different formats

Abcore Video Storyboard 22

Such as Fc fusion proteins

Abcore Video Storyboard 23

And heterodimers.

Abcore Video Storyboard 24

They are more suitable for oral delivery

Abcore Video Storyboard 25

And use in diagnostic devices.

Abcore Video Storyboard 26

They are able to pass the renal filter, resulting in their rapid blood clearance.

Abcore Video Storyboard 27

Which is advantageous when using the single-domain antibody coupled to a toxic substance.

Abcore Video Storyboard 28

They can be used as crystallization chaperones in the structure determination of challenging target proteins that would prove unsolvable using conventional strategies.

Abcore Video Storyboard 29

They can be used as introbodies to target intracellular proteins.

Abcore Video Storyboard 30

The way in which we produce single-domain antibodies for you at Abcore is as follows.

Abcore Video Storyboard 31

We start by immunizing llamas with the immunogen.

Abcore Video Storyboard 32

We use llamas because they naturally produce heavy chain-only antibodies,
which are functional antibodies devoid of light chains.

Abcore Video Storyboard 33

The llamas then go through a 90-day schedule, during which they are boosted

Abcore Video Storyboard 34

And bled to determine their specific antisera titre.

Abcore Video Storyboard 35

Once the antisera titre up

Abcore Video Storyboard 36

The peripheral blood mononuclear cells, also called PBMCs, are isolated

Abcore Video Storyboard 37

Total RNA is isolated from that

Abcore Video Storyboard 38

And cDNA is prepared.

Abcore Video Storyboard 39

The VHH genes are amplified from cDNA using proprietary PCR primers that cover the genetic diversity in this complex region.

Abcore Video Storyboard 40

The PCR product is then cloned into a phagemid vector

Abcore Video Storyboard 41

Creating the VHH Antibody Library.

Abcore Video Storyboard 42

The library is scanned through multiple rounds of affinity panning

Abcore Video Storyboard 43

Positive clones are identified via ELISA and then sequenced.

Abcore Video Storyboard 44

These clones are used to produce expressed, purified, llama monoclonal single-domain antibodies.

Abcore Video Storyboard 45

At Abcore, we give you the unique choice of selecting one of six single-domain antibody production options.

Abcore Video Storyboard 46

1: Fresh llama blood.
2: PBMCs
3: RNA and cDNA
4: SDA Antibody Library
5: Positive Clones
6: Large-Scale Production

Abcore Video Storyboard 47

For information about our pricing tiers, please enter your name, email, phone number, and select the production option you are interested in and click Submit.

Abcore Video Storyboard 48

Thank you for watching.

Abcore Video Storyboard 49

Thank you for choosing Abcore.

Video Final Deliverable:

Suggested Landing Page Design:

Abcore Landing Page

This campaign is currently live here: www.abcore-inc.com

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